CURTAIN-PTM, A WEB-BASED TOOL FOR DATA VISUALIZATION AND EXPLORATION OF MASS SPECTROMETRY-BASED PROTEOMICS (POST TRANSLATIONAL MODIFICATIONS)

To facilitate analysis and sharing of complex mass spectrometry (MS) data between ASAP Team members and external groups, we have created a tool called Curtain (http://curtain.proteo.info, http://curtainptm.proteo.info). It enables data sharing, visualization, and quantitative analysis of proteomic and post translational modification (PTM) MS data. Curtain is designed to be used by non-MS-expert end users and data for set of experiments can be shared with a simple web link. To use Curtain, the person who performs the original MS analysis uploads the acquired data as a differential analysis result file (excel or tabulated text format) that is readily obtained from all MS analysis software suites. Curtain displays the differential analysis data from each set of experiments as an interactive volcano plot. The end users can then peruse and visualize protein and/or PTM levels of selected hits in a bar chart or violin plots. Curtain also permits collections of proteins of interest, such as PD, Alzheimer’s, LRRK2 pathway, Ciliopathy, and many others to be rapidly analysed as a group. Curtain also allows facile visualization of the domain structure, AlphaFold predicted structure, and report potential interactors for each protein of interest. Curtain is a free to use and open-source resource for sharing, visualizing and exploring MS data.