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DAB Staining for GFP on Free-floating Fixed NHP Brain Tissue
Output Details
Description
This protocol details the procedure for immunohistochemical 3,3’-Diaminobenzidine (DAB) staining of free-floating fixed brain tissue sections using the avidin/biotin ABC complex to detect Green Fluorescence Protein (GFP).
This protocol has been tested with free-floating non-human primate (NHP) brain tissue that has been fixed with 4% paraformaldehyde, cryoprotected with sucrose gradients, and cryo-sectioned (50 µm).