Designing sgRNA Oligos and Inserting Guides into the GEARBOCS Vector

By Justin Savage, BSc
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Output Details

Description
This protocol describes designing sgRNAs for genes of interest using the GEARBOCS vector backbone. This method allows researchers to utilize the GEARBOCS system for astrocyte-specific genetic manipulation in CRISPR/Cas9 mice for in vivo assays
Identifier (DOI)
10.17504/protocols.io.5jyl8271rl2w/v1
Tags
  • Astrocytes
  • gRNA oligonucleotides
  • Other
  • sgRNA
  • Viral vectors
Team
Team Calakos
Program
Collaborative Research Network
Theme
Circuitry and Brain-Body Interactions

Meet the Authors

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    Justin Savage, BSc

    Key Personnel: Team Calakos, 

    Duke University

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