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Differentiation of human cortical neurons (CNs) from induced pluripotent stem cells (iPSCs) and their coculture with rat astrocytes
Output Details
Description
This protocol described the production of human cortical neurons from induced pluripotent stem cells in cocultured with commercially available rat astrocytes. The differentiation protocol of cortical neurons is adapted and modified from Shi, Y. et al., 2012 (pharmacologically directed) and Zhang, Y. et al., 2013 (transcription factor directed), as previously described by Hedegaard et.al., 2020. These neurons exhibited molecular and functional hallmarks of cortical neurons; co-culture with rat astrocytes enhanced functional activities of these neurons.
Identifier (DOI)
10.17504/protocols.io.j8nlkw6rwl5r/v1