The subthalamic nucleus (STN) is critical for behavioral control; its dysregulation consequently correlates with neurological disorders, including PD. To investigate STN and aversion, affective behavior is addressed using optogenetics in mice.
This protocol outlines steps for performing ex vivo slice whole-cell patch-clamp electrophysiology experiments, including optogenetic stimulation introduced through viral injection or transgenic expression.
This is the primary dataset for the article by Miller-Hansen et al "Thalamus orchestrates local acetylcholine-dependent dopamine release in the learning striatum"
