Fluorescent Immunolabelling for endogenous ChAT in mice striatum

By Roberta Marongiu, PhD
View Published Protocol

Output Details

Description
This immunohistochemistry protocol has been designed and adjusted specifically for ChAT immunolabelling in the mice striatum.
Identifier (DOI)
10.17504/protocols.io.8epv59wk6g1b/v1
Tags
  • Immunofluorescence
  • Immunohistochemistry
Team
Team Kaplitt
Program
Collaborative Research Network
Theme
Circuitry and Brain-Body Interactions

Meet the Authors

Related Research

Immunocytochemistry of cultured human Medium Spiny Neurons (MSNs)

This protocol describes the immunolabelling of one or several protein targets on PFA-fixed cell culture on glass coverslips.

View Protocol

Fluorescent Immunolabelling for endogenous mouse LRRK2

This protocol is used to label endogenous LRRK2 in mouse. It has been edited from West et al., 2014. Protocol optimized for fresh fixed brain tissue sectioned at 40 um.

View Protocol

Immunofluorescence imaging of cells expressing GolgiTAG (TMEM115-3HA) or HA-tagged non-Golgi annotated proteins

A method that can be used to verify the correct localisation of HA-tagged Golgi proteins by assessing their colocalization with known Golgi markers. olocalization with ACBD3.

View Protocol
View More Outputs
Aligning Science Across Parkinson's
Powered by  GDPR Cookie Compliance
Privacy Overview

This website uses cookies so that we can provide you with the best user experience possible. Cookie information is stored in your browser and performs functions such as recognising you when you return to our website and helping our team to understand which sections of the website you find most interesting and useful.