Fontana-Masson staining

By Cristian Gonzalez-Cabrera, PhD, Csilla Novák, MSc, Andres Mauricio Jaramillo Flautero, Celine Winter and Matthias Prigge, PhD
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Output Details

Description
The Fontana-Masson Staining Protocol is a detailed method for staining free-floating sections (20-50 microns) to detect melanin and other pigments in tissue samples. The procedure involves preparing a silver nitrate working solution, treating sections with triton, and sequential incubation in pre-heated silver nitrate solution, gold chloride, and thiosulfate solutions, with thorough washing steps between each incubation. The process concludes with sections being placed in PBS for mounting and counterstaining. This protocol is critical for histological studies in biology, particularly for pigment analysis in tissues.
Identifier (DOI)
10.17504/protocols.io.n92ldmxoxl5b/v1
Team
Team Vila
Program
Collaborative Research Network
Theme
Circuitry and Brain-Body Interactions

Meet the Authors

  • User avatar fallback logo

    Cristian Gonzalez-Cabrera, PhD

    Key Personnel: Team Vila, 

    Leibniz Institute for Neurobiology

  • Csilla Novák, MSc

    Key Personnel: Team Vila, 

    Leibniz Institute for Neurobiology

  • User avatar fallback logo

    Andres Mauricio Jaramillo Flautero

  • User avatar fallback logo

    Celine Winter

  • Matthias Prigge, PhD

    Co-PI (Core Leadership): Team Vila, 

    Leibniz Institute for Neurobiolog

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