Ubiquitinated proteins were enriched using Halo-m-DSK ubiquitin capture, and GFP-PINK1 complexes were isolated by IP, followed by SDS–PAGE and immunoblotting to assess PINK1 activation and downstream ubiquitination signaling upon GTPP treatment
MDFF simulations were performed to evaluate how well atomic models of mHsp60 single rings in different nucleotide states fit the in situ subtomogram averaging density maps by RMSD-based assessment of model–map agreement.
