Generation of ATG3 KO Hela cells stably expressing HaloTag-LC3B

By Xuefeng Ren, PhD
View Published Protocol

Output Details

Description
This protocol details generation of ATG3 KO Hela cells stably expressing HaloTag-LC3B.
Identifier (DOI)
10.17504/protocols.io.5qpvo3xq9v4o/v1
Tags
  • Cell biology
  • HeLa Cells
Team
Team Hurley
Program
Collaborative Research Network
Theme
PD Functional Genomics

Meet the Authors

  • User avatar fallback logo

    Xuefeng Ren, PhD

    Key Personnel: Team Hurley, 

    University of California, Berkeley

Related Research

Three-step docking by WIPI2, ATG16L1 and ATG3 delivers LC3 to the phagophore

In this manuscript, in a near-complete pathway from initial membrane recruitment to LC3 lipidation reaction, we show how a three-step targeting mechanism of the ATG12-ATG5-ATG16L1 machinery ensures a high level of regulatory control on autophagy.

View Article

Specific cell states underlie complex tissue regeneration in spiny mice

This paper compares regenerative and fibrotic healing to identify injury-induced cell states associated with persistent cell cycle progression and complex tissue regeneration in mammals.

View Article

Microscopy-based measurements of p62 recruitment in HeLa

Associated with preprint: https://www.biorxiv.org/content/10.1101/2022.11.02.514817v1

View Protocol
View More Outputs
Aligning Science Across Parkinson's
Powered by  GDPR Cookie Compliance
Privacy Overview

This website uses cookies so that we can provide you with the best user experience possible. Cookie information is stored in your browser and performs functions such as recognising you when you return to our website and helping our team to understand which sections of the website you find most interesting and useful.