Glucocerebrosidase, a Parkinson´s disease-associated protein, is imported into mitochondria and regulates complex I assembly and function

By Pascale Baden, Maria Jose Perez Jimenez, PhD, Stefanie Kalb, Hariam Raji, MSc, María Illescas, Claudio Giuliano, Federico Bertoli, MD, Alessandra Calogero, Graziella Cappelletti, Kathrin Brockmann, Thomas Gasser, Anthony Schapira, PhD, Christina Ugalde and Michela Deleidi, MD, PhD
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Output Details

Description

Raw data files used for the manuscript "Glucocerebrosidase, a Parkinson´s disease-associated protein, is imported into mitochondria and regulates complex I assembly and function" https://www.researchsquare.com/article/rs-1521848/v1

Tags
  • Dopaminergic
  • Immunofluorescence
  • Immunoprecipitation
  • iPSCs (Induced pluripotent stem cells)
  • Lentivirus
  • Live-imaging
  • Mass spectrometry
  • Microscopy
  • Midbrains
  • Proteomics
  • scRNAseq (Single-cell RNA-seq)
  • Western Blot
Team
Team Schapira
Program
Collaborative Research Network
Theme
Neuro-Immune Interactions

Meet the Authors

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    Pascale Baden

  • User avatar fallback logo

    Maria Jose Perez Jimenez, PhD

    Key Personnel: Team Schapira, 

    German Center for Neurodegenerative Diseases

  • User avatar fallback logo

    Stefanie Kalb

  • Hariam Raji, MSc

    Key Personnel: Team Schapira, 

    Institut des Maladies Génétiques Imagine

  • User avatar fallback logo

    María Illescas

  • User avatar fallback logo

    Claudio Giuliano

  • Federico Bertoli, MD

    Key Personnel: Team Schapira, 

    German Center for Neurodegenerative Diseases

  • User avatar fallback logo

    Alessandra Calogero

  • User avatar fallback logo

    Graziella Cappelletti

  • User avatar fallback logo

    Kathrin Brockmann

  • User avatar fallback logo

    Thomas Gasser

  • Anthony Schapira, PhD

    Lead PI (Core Leadership): Team Schapira, 

    University College London

  • User avatar fallback logo

    Christina Ugalde

  • Michela Deleidi, MD, PhD

    Co-PI (Core Leadership): Team Schapira, 

    Institut Imagine

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Glucocerebrosidase is imported into mitochondria and preserves complex I integrity and energy metabolism

Mutations in GBA1, the gene encoding the lysosomal enzyme β-glucocerebrosidase (GCase), which cause Gaucher’s disease, are the most frequent genetic risk factor for Parkinson’s disease (PD). Here, we employ global proteomic and single-cell…

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Collection of protocols for paper: “Glucocerebrosidase, a Parkinson´s disease-associated protein, is imported into mitochondria and regulates complex I assembly and function”

This is a collection of protocols used in a recent preprint by the Deleidi Lab, Team Schapira. You can access pre-print at https://doi.org/10.21203/rs.3.rs-1521848/v1

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Differentiation NPCs to Dopaminergic/Midbrain Neurons

This protocol details methods for differentiation of NPCs to Dopaminergic/Midbrain Neurons. This protocol is part of a Collection of protocols (dx.doi.org/10.17504/protocols.io.8epv593dng1b/v1) for the paper “Glucocerebrosidase, a Parkinson´s…

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