This website uses cookies so that we can provide you with the best user experience possible. Cookie information is stored in your browser and performs functions such as recognising you when you return to our website and helping our team to understand which sections of the website you find most interesting and useful.
Golgi immunopurification (Golgi-IP) for subcellular lipid profiling
Output Details
Description
The Golgi is a membrane-bound organelle that is central to protein and lipid processing, sorting and secretion in the cell. Despite its critical cellular function, there has been challenges to quantitatively assess Golgi lipid profiles. To overcome this hurdle, we developed a rapid harvesting and purification method using immunoprecipitation (Golgi-IP). This protocol provides details for preparing Golgi-IP lipidomics samples.
Identifier (DOI)
10.17504/protocols.io.5qpvor3dbv4o/v1