In this manuscript, in a near-complete pathway from initial membrane recruitment to LC3 lipidation reaction, we show how a three-step targeting mechanism of the ATG12-ATG5-ATG16L1 machinery ensures a high level of regulatory control on autophagy.
Protocol measures phosphoRab GTPase dephosphorylation with PPM phosphatases using purified phosphoRab GTPases. Substrates generated by phosphorylating Rab GTPases with MST3 kinase are included.
