This workflow was used to analyze a Krios dataset of the PI3KC3-C1/RAB1A Complex and generate a reconstruction of three distinct conformational states of the VPS34 lipid kinase domain.
QuPath is a bioimage analysis software designed for digital pathology and whole slide image analysis. This protocol describes how to measure CD8 density in the substantia nigra and cerebral peduncle using haematoxylin and DAB-stained brain sections.
Extensive image processing techniques were used to analyze cellular mechanisms, including deconvolution, machine learning, and manual identification of structures. Creative approaches were employed to ensure reliable and reproducible results.
