A HeLa cell line expressing PINK1 fused to GFP from the endogenous locus was generated using CRISPR–Cas9 nickase–mediated genome editing with homology-directed repair. GFP-positive clones were isolated by fluorescence-activated cell sorting.
A data-driven molecular map of the DLPFC reveals distinct spatial domains and cell populations, offering insights into neuropsychiatric disorders. The study provides a roadmap for implementing spatial clustering approaches in the human brain.
