In vitro synthesis of PE2 (nCas9-MMLV RT fusion) polyA mRNA using T7 RNA polymerase

By Donald Rio, PhD
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Output Details

Description

We describe the preparation of synthetic capped and polyadenylated messenger RNA (mRNA) encoding a Cas9 nickase-reverse transcriptase fusion protein (PE2) for use in genome editing with a method called prime editing.

Identifier (DOI)
10.17504/protocols.io.b3fmqjk6
Tags
  • Biochemistry
  • Cas9
  • Genome editing
  • In Vitro
  • mRNA
  • PolyA tailing
  • Purification
Team
Team Rio
Program
Collaborative Research Network
Theme
PD Functional Genomics

Meet the Authors

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