Indirect Proximity Ligation Assay (PLA) – Brightfield

By Ayse Ulusoy, PhD, Rita Pinto-Costa, Angela Rollar, MSc and Donato Di Monte, MD
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Output Details

Description
Indirect Proximity Ligation Assay (PLA) is a powerful molecular technique used to detect and visualize protein-protein interactions, protein modifications, and protein complex formations within cells or tissues. This method is based on the principles of proximity-dependent ligation and utilizes specific antibodies to detect the nitration of proteins on free-floating brain sections. Here we describe the PLA protocol that we routinely use in our laboratory to detect nitrated alpha-synuclein and nitration of mitochondrial enzymes such as SOD2 and the mitochondrial complex 1 subunit NDUFB8.
Identifier (DOI)
10.17504/protocols.io.bp2l6x94klqe/v1
Tags
  • Protein-protein interaction
Team
Team Schapira
Program
Collaborative Research Network
Theme
Neuro-Immune Interactions

Meet the Authors

  • Ayse Ulusoy, PhD

    Key Personnel: Team Schapira, 

    German Center for Neurodegenerative Diseases

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    Rita Pinto-Costa

  • User avatar fallback logo

    Angela Rollar, MSc

    Key Personnel: Team Schapira, 

    German Center for Neurodegenerative Diseases

  • Donato Di Monte, MD

    Co-PI (Core Leadership): Team Schapira, 

    German Center for Neurodegenerative Diseases

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