Lentiviral vector plasmid for overexpression of ATP10B (E993A)

By Chris Van den Haute, PhD and Peter Vangheluwe, PhD
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Output Details

Description
Lentiviral vector plasmid for overexpression of ATP10B (E993A) validated for use in cell culture
Team
Team Vangheluwe
Program
Collaborative Research Network
Theme
PD Functional Genomics

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The lipid flippase ATP10B enables cellular lipid uptake under stress conditions

ATP10B mutations are linked to Parkinson's and Lewy body disease. ATP10B acts as a lipid transporter in late endo-/lysosomes, enhancing phosphatidylcholine uptake in cells under stress conditions like rotenone treatment.

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Image Processing and 3D Reconstruction – Chen et al. 2025 NSMB

Image processing and 3D reconstruction applied in Chen et al. 2025 NSMB

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Plasmid construction – Chen et al. 2025 NSMB

Standard cloning procedure.

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