Live-cell imaging for synaptic vesicle precursors in human iNeuron axons

By Dan Dou and Erika Holzbaur, PhD
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Output Details

Description
Here, we describe procedure and equipment used for live-imaging of synaptic vesicle precursors. This was performed using DIV21 human iPSC-derived excitatory glutamatergic neurons. Equipment and software used varied based on scheduled upgrades to microscopy equipment during the course of this study.
Identifier (DOI)
10.17504/protocols.io.5jyl8p9mdg2w/v1
Tags
  • Fluorescence imaging
  • Human
  • In Cellulo
  • Microscopy - optical
  • Neurons
  • Synaptic trafficking
Team
Team Hurley
Program
Collaborative Research Network
Theme
PD Functional Genomics

Meet the Authors

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Aligning Science Across Parkinson's
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