Localised axotomy of human Cortical Neurons (CNs) from induced pluripotent stem cells (iPSCs)

By Quyen Do, Frederico Nebuloni and Richard Wade-Martins, PhD
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Output Details

Description
This protocol describes the process followed to perform localised axotomy of iPSC-derived human cortical axons cultured within fluid-walled dumbbells in 6 cm TCT-treated Petri dishes. A similar protocol was first described by Soitu et al., 2020 to perform wounds assays in monolayer cell cultures.
Identifier (DOI)
10.17504/protocols.io.rm7vzbp14vx1/v1
Tags
  • Axons
  • Human
  • In Vitro
  • iPSC
  • iPSCs (Induced pluripotent stem cells)
  • Microfluidic devices
Team
Team Cragg
Program
Collaborative Research Network
Theme
Circuitry and Brain-Body Interactions

Meet the Authors

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