LRRK2 disrupts the number and composition of active zone release sites in vulnerable dopamine axons
Output Details
Description
We used a previously described intersectional/subtractive approach that allows for simultaneous labeling of Aldh1a1+ with EYFP and Aldh1a1- neuronal with mCherry, or Anxa,1+ with mCherry, respectively. Striatal brain sections were then stained with antibodies against bassoon to analyze the number and organization of active zone sites in Aldh1a1+ (EYFP+) or Aldh1a1- (mCherry+) axons from the same mouse. Due to the high signal of bassoon in the striatum, we used structured illumination microscopy (SIM) for improved resolution, followed by 3D reconstruction analysis.
Identifier (DOI)
10.6019/S-BIAD2071
