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Measurement of GLP-1 release in cell supernatant from Hutu-80 enteroendocrine cells via ELISA
Output Details
Description
This protocol was adapted from Cat. # EGLP-35K to optimise the seeding density of the assay based on the cell line used (Hutu 80 enteroendocrine cells RRID: CVCL_1301), moreover, cell line used and media conditions used to characterise GLP-1release.
The lowest level of GLP-1 that can be detected by this assay is 2 pM (100ul plasma sample size).
Identifier (DOI)
10.17504/protocols.io.j8nlkokm6v5r/v1