Neuromelanin staining (Fontana-Masson staining)+ TH-DAB staining on midbrain organoids

By Michela Deleidi, MD, PhD, Federico Bertoli, MD and Hariam Raji, MSc
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Output Details

Description
Fontana-Masson staining is a silver staining technique that is commonly used to identify melanin-containing cells. We combined it with DAB-TH staining that works by using an antibody to detect the presence of TH, followed by a reaction with a substrate (DAB) that results in the formation of a brown-colored product at the site of the antigen-antibody interaction.
Identifier (DOI)
10.17504/protocols.io.5jyl8jon8g2w/v1
Tags
  • Neuromelanin
  • Staining
Team
Team Schapira
Program
Collaborative Research Network
Theme
Neuro-Immune Interactions

Meet the Authors

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