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Oxford Nanopore Technologies (ONT) library preparation and sequencing of DNA prepared using droplet Multiple Displacement Amplification (dMDA)
Output Details
Description
Debranching of DNA generated by Multiple Displacement Amplification (MDA) is an essential step prior to nanopore sequencing. This protocol describes an optimised T7-based debranching protocol for genomic DNA amplified from single nuclei using droplet MDA (dMDA) obtained according to Kalef-Ezra et al 2023 (https://www.protocols.io/view/manual-isolation-of-nuclei-from-human-brain-using-kxygxzjjov8j/v1). Subsequent library preparation and sequencing steps are also outlined here.
Identifier (DOI)
10.17504/protocols.io.q26g71x2qgwz/v1