pCAG-FIP200 (1-640)-L324D/R543D/R548D-TSF

View Published Lab Material

Output Details

Description
Mammalian expression of FIP200 NTD mutant
Team
Team Hurley
Program
Collaborative Research Network
Theme
PD Functional Genomics

Related Research

Structure and activation of the human autophagy-initiating ULK1C:PI3KC3-C1 supercomplex

Authors determine cryo-EM structure of human ULK1C core and its complex with PI3KC3-C1. ULK1C core undergoes a rearrangement from 2:1:1 to 2:2:2 stoichiometry, suggesting a structural mechanism for autophagy initiation.

View Article

How Membrane Contact Sites Shape the Phagophore

Phagophores form membrane contact sites with organelles like the vacuole, ER, and lipid droplets. In situ imaging techniques, such as cryo-CLEM, provide valuable insights into these interactions, aiding in understanding autophagosome biogenesis.

View Article

FISH CODETECTION

DIG and Fluorescein labeled riboprobes Fluorescent in situ hybridization Frozen brain sections

View Protocol
View More Outputs