Polyamines are crucial for brain function. ATP13A4 is the main polyamine transporter in astrocytes, impacting astrocyte morphology, synapse formation, and neurodevelopment. Mutations in ATP13A4 are linked to neurodevelopmental disorders.
P4-ATPase lipid flippases create lipid asymmetry in cells. A new strategy using NBD-lipid uptake assays improves sensitivity and analysis of ATP11C function. This method enhances studying regulatory interactions in mammalian cells.
