Piggybac-mediated stable expression of NGN2 in iPSCs for differentiation into excitatory glutamatergic neurons V.2

Description

We adapted a previously-described method (Pantazis et al., 2022) for employing Piggybac transfection to stably express doxycycline-inducible NGN2 in human iPSCs. After stable integration of NGN2, proceed to differentiate iPSCs using protocol “iNeuron differentiation from human iPSCs.”

Identifier (DOI)

10.17504/protocols.io.e6nvwj54dlmk/v1

Tags

2D cultures
iNeurons (induced neurons)
Transfection

Team

Team Hurley

Program

Collaborative Research Network

Theme

PD Functional Genomics

Meet the Authors

Dan Dou

Key Personnel: Team Hurley,

University of Pennsylvania
Alexander Boecker, MD

Key Personnel: Team Hurley,

University of Pennsylvania
Erika Holzbaur, PhD

Co-PI (Core Leadership): Team Hurley,

University of Pennsylvania

Piggybac-mediated stable expression of NGN2 in iPSCs for differentiation into excitatory glutamatergic neurons V.2

Output Details

Meet the Authors

Dan Dou

Alexander Boecker, MD

Erika Holzbaur, PhD

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