Piggybac-mediated stable expression of NGN2 in iPSCs for differentiation into excitatory glutamatergic neurons V.2

By Dan Dou, Alexander Boecker, MD and Erika Holzbaur, PhD
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Output Details

Description
We adapted a previously-described method (Pantazis et al., 2022) for employing Piggybac transfection to stably express doxycycline-inducible NGN2 in human iPSCs. After stable integration of NGN2, proceed to differentiate iPSCs using protocol “iNeuron differentiation from human iPSCs.”
Identifier (DOI)
10.17504/protocols.io.e6nvwj54dlmk/v1
Tags
  • 2D cultures
  • iNeurons (induced neurons)
  • Transfection
Team
Team Hurley
Program
Collaborative Research Network
Theme
PD Functional Genomics

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