Protein quantification by SDS-PAGE and Coomassie staining

Research Community
Research Community

ASAP supports three programs and serves as a member of multiple consortia to advance research for the benefit of the global Parkinson’s disease community.
Explore
Highlights
Highlights

ASAP shares the impact that our initiative, network, and supported programs have had on providing new insights into Parkinson’s disease. Discover news, interviews, awards, and annual impact.
Explore
Open Science
Open Science

ASAP is dedicated to facilitating a research environment where meaningful collaboration, research-enabling resources, and data sharing provides the answers we need to understand, diagnose, and treat Parkinson’s disease. Discover our open science philosophy.
Explore
Tools & Resources
Tools & Resources

ASAP is committed to developing and openly sharing research outputs, including data, code, protocols, and key lab materials, to help the scientific community build upon existing work.
Explore

Description

This protocol provides a step-by-step guide for quantifying protein concentration by SDS-PAGE and Coomassie staining. It details the preparation of samples and BSA standards, protein separation via electrophoresis, staining, imaging, and data analysis for accurate protein quantification.

Identifier (DOI)

10.17504/protocols.io.kqdg3qqqev25/v1

Discovery Team

Team Vangheluwe

Program

Collaborative Research Network

Theme

PD Functional Genomics

Protein quantification by SDS-PAGE and Coomassie staining

Output Details

Related Research

Astrocytic polyamine transport by ATP13A4 tunes excitatory synaptic transmission

all protocols related to “Spermidine suppresses glial inflammation and parkinsonian abnormalities in ATP13A2 deficiency”

Fluorescence-Gated Flow Cytometry Approach for Measuring Lipid Flippase Activity in Mammalian Cells