Protocol for Neuronal Live-imaging of primary cultures

Research Community
Research Community

ASAP supports three programs and serves as a member of multiple consortia to advance research for the benefit of the global Parkinson’s disease community.
Explore
Highlights
Highlights

ASAP shares the impact that our initiative, network, and supported programs have had on providing new insights into Parkinson’s disease. Discover news, interviews, awards, and annual impact.
Explore
Open Science
Open Science

ASAP is dedicated to facilitating a research environment where meaningful collaboration, research-enabling resources, and data sharing provides the answers we need to understand, diagnose, and treat Parkinson’s disease. Discover our open science philosophy.
Explore
Tools & Resources
Tools & Resources

ASAP is committed to developing and openly sharing research outputs, including data, code, protocols, and key lab materials, to help the scientific community build upon existing work.
Explore

Description

Neurons stand out as remarkably intricate cells. The development of live-imaging techniques on primary neurons expressing genetically engineered biosensor has proven pivotal in advancing our comprehension of neuronal function at both cellular and molecular level. This protocol introduces a concise method for live-imaging neuronal activity.

Identifier (DOI)

10.17504/protocols.io.q26g7pn4qgwz/v1

Team

Team De Camilli

Program

Collaborative Research Network

Theme

PD Functional Genomics

Meet the Authors

Camila Pulido, PhD

Key Personnel: Team De Camilli,

Weill Cornell Medicine
Timothy Ryan, PhD

Co-PI (Core Leadership): Team De Camilli,

Cornell University

Protocol for Neuronal Live-imaging of primary cultures

Output Details

Meet the Authors

Camila Pulido, PhD

Timothy Ryan, PhD

Related Research

Western blot source data

Immunofluorescence source data

Alphafold structural predictions