Raw band intensity values for ATG3 WT or Mutants in vitro LC3 lipidation

By Xuefeng Ren, PhD and James Hurley
View Published Dataset

Output Details

Description
Raw band intensity values used for the quantification of in vitro LC3 lipidation results (Fig4C) in Three-step docking by WIPI2, ATG16L1 and ATG3 delivers LC3 to the phagophore.
Identifier (DOI)
10.1101/2023.07.17.549391
Tags
  • ATG12-5-16L1
  • ATG3 (E2-like enzyme)
  • Human
  • In Vitro
  • LC3
  • LC3 lipidation
  • Other
Team
Team Hurley
Program
Collaborative Research Network
Theme
PD Functional Genomics

Meet the Authors

Related Research

Three-step docking by WIPI2, ATG16L1 and ATG3 delivers LC3 to the phagophore

In this manuscript, in a near-complete pathway from initial membrane recruitment to LC3 lipidation reaction, we show how a three-step targeting mechanism of the ATG12-ATG5-ATG16L1 machinery ensures a high level of regulatory control on autophagy.

View Article

Jensen_etal_2022_membrane_tube_images

Membrane tube assay data for Jensen et al, 2022

View Dataset

Membrane curvature sensing and stabilization by the autophagic LC3 lipidation machinery

During autophagy initiation, the curved phagophore is stabilized. Using in vitro reconstitution and MD simulations, authors show that WIPI2 and ATG16L1 bind these curved phagophores and the ATG12-5-16L1 complex is responsible for membrane curvature.

View Article
View More Outputs