Single cell analysis of iPSC-derived midbrain organoids

By Pascale Baden, Maria Jose Perez Jimenez, PhD, Stefanie Kalb, Hariam Raji, María Illescas, Claudio Giuliano, Federico Bertoli, MD, Alessandra Calogero, Graziella Cappelletti, Kathrin Brockmann, Thomas Gasser, Anthony Schapira, PhD, Cristina Ugalde and Michela Deleidi, MD, PhD

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Description

The following script was used for analysis of gene corrected (GC) versus GBA1 mutant (MUT) midbrain organoids. The purpose was to combine, filter, integrate, and identify clusters and differentially expressed genes sets. This is part of a Collection of protocols (dx.doi.org/10.17504/protocols.io.8epv593dng1b/v1) for the paper "Glucocerebrosidase, a Parkinson´s disease-associated protein, is imported into mitochondria and regulates complex I assembly and function" (https://doi.org/10.21203/rs.3.rs-1521848/v1)

Identifier (DOI)

10.5281/zenodo.6475864

Tags

Midbrains
Organoid
scRNAseq (Single-cell RNA-seq)
Sequencing

Team

Team Schapira

Program

Collaborative Research Network

Theme

Neuro-Immune Interactions

Meet the Authors

Pascale Baden
Maria Jose Perez Jimenez, PhD

Key Personnel: Team Schapira,

German Center for Neurodegenerative Diseases
Stefanie Kalb
Hariam Raji
María Illescas
Claudio Giuliano
Federico Bertoli, MD

Key Personnel: Team Schapira,

German Center for Neurodegenerative Diseases
Alessandra Calogero
Graziella Cappelletti
Kathrin Brockmann
Thomas Gasser
Anthony Schapira, PhD

Lead PI (Core Leadership): Team Schapira,

University College London
Cristina Ugalde
Michela Deleidi, MD, PhD

Co-PI (Core Leadership): Team Schapira,

Institut Imagine

Single cell analysis of iPSC-derived midbrain organoids

Output Details

Meet the Authors

Pascale Baden

Maria Jose Perez Jimenez, PhD

Stefanie Kalb

Hariam Raji

María Illescas

Claudio Giuliano

Federico Bertoli, MD

Alessandra Calogero

Graziella Cappelletti

Kathrin Brockmann

Thomas Gasser

Anthony Schapira, PhD

Cristina Ugalde

Michela Deleidi, MD, PhD

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Collection of protocols for paper: “Glucocerebrosidase, a Parkinson´s disease-associated protein, is imported into mitochondria and regulates complex I assembly and function”