SNP Genotyping and ApoE Genotyping

By Huw Morris, Manuela Tan, Donald Grosset and Nigel Williams
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Output Details

Description
This protocol details the steps for DNA extraction from a human blood sample, quality control, and SNP and APOE genotyping. The protocol has been adapted from the PRoBaND SNP Genotyping and ApoE Genotyping Protocol: Malek, N. et al (2015). Tracking Parkinson’s: Study Design and Baseline Patient Data. Journal of Parkinson’s Disease, 5(4), 947–959. https://doi.org/10.3233/JPD-150662
Identifier (DOI)
10.17504/protocols.io.by9ypz7w
Tags
  • APOE (Apolipoprotein E)
  • Blood
  • DNA
  • Genotyping
  • Parkinson's disease
  • SNPs (Single Nucleotide Polymorphisms)
Team
Team Hardy
Program
Collaborative Research Network
Theme
PD Functional Genomics

Meet the Authors

  • Huw Morris

    Collaborating PI: Team Hardy, 

    University College London

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    Manuela Tan

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    Donald Grosset

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    Nigel Williams

Related Research

huw-morris-lab/PDD_GWSS

The manuscript by Real et al. investigates the relationship between LRP1B and APOE loci and the onset of Parkinson’s disease dementia, utilizing specific code for analysis.

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Combining biomarkers for prognostic modelling of Parkinson’s disease

Parkinson's disease progression varies among patients. Predicting progression accurately is crucial for clinical trial selection. Blood biomarkers like serum NfL, along with genetic factors (GBA, APOE) can enhance prediction beyond age and phenotype.

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Association between the LRP1B and APOE loci and the development of Parkinson’s disease dementia

Genetic analysis of 3,964 PD cases revealed APOE-ϵ4 allele and new loci as risk factors for PD dementia progression, implicating amyloid pathway in PDD development and potential for amyloid-targeting therapy.

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