Stereotaxic Optic Fiber Implantation in Mice

Output Details

This protocol describes a procedure to implant one or multiple optic fiber cannulae in mouse brains used for optical recording brain activity using fiber photometry or for optogenetic stimulation or inhibition of neuronal activity in mice performing behavioral tasks. Typically, this procedure is performed on mice expressing indicators for neuronal activity, such as GCaMP8, or opsins, such as Channelrhodopsin2, which were introduced through viral injection or transgenic expression.
Tags
  • Animal
  • Fiber photometry
  • In Vivo
  • Mouse
  • Optogenetics
  • Stereotactic
  • Surgical

Meet the Authors

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    Richard Roth, PhD

    Key Personnel: Team Surmeier

    Stanford University

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    Jun Ding, PhD

    Collaborating PI: Team Surmeier

    Stanford University