Targeted long-read RNA-seq

Output Details

Synapse Accession Number (Project SynID): syn53642785 Raw long read RNAseq data generated and used in the article “The annotation of GBA1 has been concealed by its protein-coding pseudogene GBAP1”. Targeted long read RNAseq of SNCA, GBA1 and GBAP1. The sequencing data is PacBio Sequel IIe targeted Iso-Seq where enrichment was performed using hybridization probes. Three datasets available: (i) human brain (ii) iPSC-derived astrocytes microglia neurons and (iii) iPSC-derived midbrain dopaminergic neurons.
Identifier (DOI)
10.7303/syn53642785
Tags
  • Analysis
  • Cell culture
  • GBA1
  • GBAP1
  • GCase activity
  • Gene-annotation
  • Human
  • Human brains
  • Immunofluorescence
  • iPSCs (Induced pluripotent stem cells)
  • Long-read sequencing
  • Other
  • PacBio Iso-Seq preparation
  • Postmortem
  • Protein-coding pseudogene
  • snRNA-seq (Single-nuclear RNA-seq)
  • Transcripts
  • Transfection
  • Western Blot

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