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Tissue processing and sectioning — Oscar Moreno Ramos and Divya Raj

By Divya Darwinarulseeli, Oscar Moreno Ramos, Rajeshwar Awatramani, PhD
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Output Details

Description
This protocol describes mouse brain fixation, sucrose cryoprotection, freezing, and sectioning before immunofluorescence on free-floating sections. It retains the explanations, practical instructions, and handling notes from the supplied protocol. The detailed sectioning procedure applies to P14 and older mouse brain samples. 
Identifier (DOI)
10.17504/protocols.io.byl42ex5rvo5/v1
Tags
  • Immunofluorescence
  • Other
  • Sectioning
Discovery Team
Team Dombeck, Team Awatramani
Program
Collaborative Research Network
Theme
Circuitry and Brain-Body Interactions PD Heterogeneity

Meet the Authors

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