Ubiquitin immunoprecipitation using an anti-ubiquitin nanobody
Output Details
Description
This protocol describes a method to detect ubiquitination on a protein of interest. This technique relies on immunoprecipitation (IP) of ubiquitinated proteins from a cell lysate through the use of an anti-ubiquitin nanobody coupled to agarose beads. The eluate can be run on SDS-PAGE to determine whether the protein of interest was recovered in the IP and, therefore, ubiquitinated.
Identifier (DOI)
10.17504/protocols.io.dm6gp36k1vzp/v1
