Western blotting for LRRK2 signalling in macrophages

By Susanne Herbst, PhD
View Published Protocol

Output Details

Description

This protocol describes the immunoblotting for components of the LRRK2 signalling pathway (LRRK2, LRRK2 pS935 and phospho-Rabs) using Invitrogen NuPage SDS-PAGE reagents and the BioRad Turbo Blot transfer system.

Identifier (DOI)
10.17504/protocols.io.3byl4k67jvo5/v1
Tags
  • Cell culture
  • LRRK2
  • Phosphorylation
  • Rab GTPases
  • Western Blot
Team
Team Hardy
Program
Collaborative Research Network
Theme
PD Functional Genomics

Meet the Authors

  • Susanne Herbst, PhD

    Key Personnel: Team Hardy, 

    Royal Veterinary College

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