Whole-cell patch-clamp recordings

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Description

This protocol describes the preparation of hippocampal slices from mice for whole-cell patch-clamp recordings. Whole-cell recordings are made from CA1 pyramidal neurons, with AMPAR-mediated EPSCs evoked by stimulation in the stratum radiatum and monitored under voltage-clamp conditions.

Identifier (DOI)

10.17504/protocols.io.dm6gpmnxpgzp/v1

Discovery Team

Team Edwards

Program

Collaborative Research Network

Theme

Circuitry and Brain-Body Interactions

Meet the Authors

Akio Mori

External Collaborator
Hongfei Xu

External Collaborator
Robert Edwards, MD

Lead PI (Core Leadership): Team Edwards,

University of California, San Francisco

Whole-cell patch-clamp recordings

Output Details

Meet the Authors

Akio Mori

Hongfei Xu

Robert Edwards, MD

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