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  • Tet-off TauRD (HEK293T)

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    Associated with the following preprint: Saha et al., 2022 (published on biorxiv on Feb 19th, 2022) https://www.biorxiv.org/content/10.1101/2022.02.18.481043v1.full

  • H9 ES AAVS1-NGN2 FAM134A-/-; PiggyBac-Keima-RAMP4

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    ES cells were modified to create iNeurons lacking FAM134A and expressing Keima-RAMP4 ER-phagy flux reporter. CRISPR/Cas9 was used to introduce NEUROG2 construct in AAVS1 locus. Transfected with Keima-RAMP4 vector.

  • iPS Cell line CRICKi011-A (iFCI016), c.G152A mutation in Exon 3 of SNCA

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    Mutations in the SNCA gene cause rare autosomal dominant Parkinson’s disease. iPSC lines were created from individuals with SNCA G51D mutations, showing potential for disease modeling and therapy development for synucleinopathies.

  • HeLa S3 penta KO-ATG14 KO

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    Cell Line: ID HeLa S3 penta KO-ATG14 KO cell line.

  • iPS Cell line: CRICKi012-A (iFCI017), c.G152A mutation in Exon 3 of SNCA

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    Mutations in SNCA gene cause rare Parkinson’s disease. iPSC lines from individuals with SNCA G51D mutation were generated successfully, showing normal characteristics. These iPSC lines can aid in studying synucleinopathies for potential therapies.

  • HeLa S3 penta KO-TBK1 KO

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    Cell Line: ID HeLa S3 penta KO-TBK1 KO cell line.

  • H9 ES AAVS1-NGN2 FAM134C-/-

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    ES cells manipulated with CRISPR/Cas9 lack FAM134C receptor for ER-phagy. NEUROG2 construct introduced via AAVS1 locus in human embryonic stem cells, creating iNeurons. Derived from blastocyst stage, female, Homo sapiens.

  • Tet-off FLTau (HEK293T)

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    Associated with the following preprint: Saha et al., 2022 (published on biorxiv on Feb 19th, 2022) https://www.biorxiv.org/content/10.1101/2022.02.18.481043v1.full

  • HeLa S3 penta KO-TBK1/ULK1/ULK2 TKO

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    Cell Line: HeLa S3 penta KO-TBK1/ULK1/ULK2 TKO cell line.

  • HEK293 FIP200-/- TMEM192-HA ::mNEON-YIPF4

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    HEK293 FIP200-/- TMEM192-HA ::mNEON-YIPF4

  • H9 ES AAVS1-NGN2 TEX264-/-; PiggyBac-Keima-REEP5

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    ES cells modified with CRISPR/Cas9 to lack TEX264, express Keima-REEP5 ER-phagy flux reporter, NEUROG2, and mKeima fluorescent protein. Derived from human embryonic stem cells at the blastocyst stage.

  • H9 ES AAVS1-NGN2 FLAG-EEA1::TMEM192-HA

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    ES cells were modified to produce iNeurons expressing FLAG-tagged EEA1 and TMEM192-HA. NEUROG2 construct was inserted into AAVS1 locus using CRISPR/Cas9. EEA1 was N-terminally tagged with a 3xFLAG tag.

  • H9 ES AAVS1-NGN2 FAM134C/A/B-/-;TEX264-/-; CCPG1-/-; PiggyBac-Keima-REEP5

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    ES cells were modified to produce iNeurons lacking ER-phagy receptor genes and expressing Keima-REEP5 reporter. CRISPR/Cas9 was used to introduce NEUROG2 construct in AAVS1 locus, and various genes were knocked out.

  • H9 ES AAVS1-NGN2 TEX264-/-; PiggyBac-Keima-RAMP4

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    ES cells were modified using CRISPR/Cas9 to lack TEX264 and express Keima-RAMP4 ER-phagy flux reporter. NEUROG2 construct was introduced in AAVS1 locus. Cells are human embryonic stem cells with a fluorescent protein marker.

  • H9 ES AAVS1-NGN2 FAM134C/A/B-/-; PiggyBac-Keima-RAMP4

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    ES cells were modified to produce iNeurons lacking FAM134C, A, B receptors and expressing Keima-RAMP4 ER-phagy flux reporter. CRISPR/Cas9 was used to introduce NEUROG2 construct and knockout RETREG1, 2, 3 genes.

  • H9 ES AAVS1-NGN2 FAM134C/A/B-/-

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    ES cells modified using CRISPR/Cas9 to lack ER-phagy receptor FAM134C, A & B. Introduced NEUROG2 gene in AAVS1 locus. Derived from human embryonic stem cells at blastocyst stage.

  • HeLa ::TMEM192-HA YIPF4-/-

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    HeLa ::TMEM192-HA YIPF4-/- PubMed=37757899; Characteristics: Using CRISPR/Cas9 TMEM192 was C-terminally tagged on both alleles with a 3xHA epitope (from parent cell line). Knockout cell: Method=CRISPR/Cas9; HGNC; 28145; YIPF4. Transformant: NCBI_TaxID; 28285; Adenovirus 5. Derived from site: In situ; Fetal kidney; UBERON=UBERON_0002113. NCBI_TaxID=9606; ! Homo sapiens (Human) RRID:CVCL_C0I5 ! HEK293::TMEM192-3xHA Female Fetus Transformed cell line

  • H9 ES AAVS1-NGN2 FAM134B-/-; PiggyBac-Keima-RAMP4

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    ES cells modified to lack FAM134B and express Keima-RAMP4 ER-phagy reporter using CRISPR/Cas9. NEUROG2 construct added to AAVS1 locus. Transfected with SERP1 and mKeima. Derived from human embryonic stem cells at blastocyst stage.

  • H9 ES AAVS1-NGN2 FAM134B-/-

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    Cell Line: ES cells for making iNeurons lacking the ER-phagy receptor FAM134B.

  • A549 PIP4P1 (TMEM55B) KO (CVCL_D3VW)

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    A549 PIP4P1 (TMEM55B) CRISPR/CAS9 A549 knock-out cell line generated from parental A549 cells (ATCC A549 CCl-185) using CRISPR/Cas9 gene-editing technology. The cell line was verified by immunoblotting analysis and DNA-sequencing analysis. Used in publication: doi: https://doi.org/10.1101/2023.06.07.544051

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