HEK293::TMEM192-3xHA RB1CC1-/- mNEON-YIPF4
By onHEK293::TMEM192-3xHA RB1CC1-/- mNEON-YIPF4 PubMed=37757899; Characteristics: Using CRISPR/Cas9 TMEM192 was C-terminally tagged on both alleles with a 3xHA epitope (from parent cell line). Characteristics: Using CRISPR/Cas9 YIPF4 was N-terminally tagged on both alleles with mNeonGreen (PubMed=37757899). Knockout cell: Method=CRISPR/Cas9; 15574; RB1CC1. Transfected with: UniProtKB; A0A1S4NYF2; mNeonGreen (derivative of Branchiostoma lanceolatum blFP-Y3). Transformant: NCBI_TaxID; 28285; Adenovirus 5. Derived from site: In situ; Fetal kidney; UBERON=UBERON_0002113. NCBI_TaxID=9606; ! Homo sapiens (Human) RRID:CVCL_D1KQ ! HEK293::TMEM192-3xHA RB1CC1-/- Female Fetus
HEK293::TMEM192-3xHA RB1CC1-/-
By onHEK293::TMEM192-3xHA RB1CC1-/- PubMed=37757899; Characteristics: Using CRISPR/Cas9 TMEM192 was C-terminally tagged on both alleles with a 3xHA epitope (from parent cell line). Knockout cell: Method=CRISPR/Cas9; 15574; RB1CC1. Transformant: NCBI_TaxID; 28285; Adenovirus 5. Derived from site: In situ; Fetal kidney; UBERON=UBERON_0002113. NCBI_TaxID=9606; ! Homo sapiens (Human) RRID:CVCL_C0I5 ! HEK293::TMEM192-3xHA Female Fetus Transformed cell line
HeLa::TMEM192-3xHA
By onHeLa ::TMEM192-3xHA PubMed=37757899; Population: African American. Characteristics: Using CRISPR/Cas9 TMEM192 was C-terminally tagged on both alleles with a 3xHA epitope (from parent cell line). Transformant: NCBI_TaxID; 333761; Human papillomavirus type 18 (HPV18). Derived from site: In situ; Uterus, cervix; UBERON=UBERON_0000002. NCIt; C27677; Human papillomavirus-related cervical adenocarcinoma NCBI_TaxID=9606; ! Homo sapiens (Human) RRID:CVCL_0030 ! HeLa Female 30Y6M Cancer cell line
HEK293E_Clu TL1
By onCell Line: Transfected with the pB-T-PAF expression plasmid expressing CLU under the tet operator and the PB-RN reverse transactivator plasmid expressing the reverse tetracycline-controlled transactivator under the CMV promoter.
HEK293E_Clu WT
By onCell Line: Transfected with the pB-T-PAF expression plasmid expressing CLU under the tet operator and the PB-RN reverse transactivator plasmid expressing the reverse tetracycline-controlled transactivator under the CMV promoter.
HEK293T TetOff-SNCA-A53T mNeonGreen-3K-1-10-IRES-mKate2
By onID HEK293T TetOff-SNCA-A53T mNeonGreen-3K-1-10-IRES-mKate2 AC CVCL_D4BZ SY Syn10 split mNeonGreen CC Characteristics: Transduced with an all-in-one TetOff construct for the controllable expression of untagged SNCA p.Ala53Thr (from parent cell line). CC Characteristics: Additional expression of a plasmid encoding a bicistronic IRES construct of a split mNeonGreen (3K variant, B-strands 1-10) as well as an mKate2 marker (Direct_author_submission). CC Transfected with: HGNC; 11138; SNCA (with p.Ala53Thr). CC Transfected with: UniProtKB; P04483; Escherichia coli Tn10 tetR. CC Transfected with: UniProtKB; P06492; Human herpesvirus 1 VP16 (UL28) (with p.Met1_Arg360del). CC Transfected with: mKate2, a bright red fluorescent protein derived from eqFP578. CC Transfected with: UniProtKB; A0A1S4NYF2; mNeonGreen (derivative of Branchiostoma lanceolatum blFP-Y3). CC Transfected with: UniProtKB; P0C2P0; Aspergillus terreus blasticidin-S deaminase (Bsd). CC Transfected with: UniProtKB; P13249; S.alboniger pac (PuroR). CC Transfected with: UniProtKB; P00552; Transposon Tn5 neo. CC Transformant: NCBI_TaxID; 28285; Adenovirus 5. CC Transformant: NCBI_TaxID; 1891767; Simian virus 40 (SV40) . CC Miscellaneous: Cell line information from personal communication of Yuste-Checa P. CC Derived from site: In situ; Fetal kidney; UBERON=UBERON_0002113. OX NCBI_TaxID=9606; ! Homo sapiens (Human) hi CVCL_D4BW ! HEK293T TetOff-SNCA-A53T SX Female AG Fetus CA Transformed cell line Parental cell line: HEK293T (RRID:CVCL_0063)
HEK293T TetOff-SNCA-A53T EGFP-RNF152-IRES-mScarlet-I
By onID HEK293T TetOff-SNCA-A53T EGFP-RNF152-IRES-mScarlet-I AC CVCL_D4BY SY Syn10 RNF152 reporter CC Characteristics: Transduced with an all-in-one TetOff construct for the controllable expression of untagged SNCA p.Ala53Thr (from parent cell line). CC Characteristics: Additional expression of a plasmid encoding a bicistronic IRES construct of EGFP-tagged RNF152, along with an mScarlet-I transfection marker (Direct_author_submission). CC Transfected with: HGNC; 26811; RNF152. CC Transfected with: HGNC; 11138; SNCA (with p.Ala53Thr). CC Transfected with: UniProtKB; P04483; Escherichia coli Tn10 tetR. CC Transfected with: UniProtKB; P06492; Human herpesvirus 1 VP16 (UL28) (with p.Met1_Arg360del). CC Transfected with: UniProtKB; P42212; GFP (with p.Phe64Leu, p.Ser65Thr and p.His231Leu = EGFP). CC Transfected with: mScarlet, a red fluorescent protein. CC Transfected with: UniProtKB; P0C2P0; Aspergillus terreus blasticidin-S deaminase (Bsd). CC Transfected with: UniProtKB; P00552; Transposon Tn5 neo. CC Transformant: NCBI_TaxID; 28285; Adenovirus 5. CC Transformant: NCBI_TaxID; 1891767; Simian virus 40 (SV40) . CC Miscellaneous: Cell line information from personal communication of Yuste-Checa P. CC Derived from site: In situ; Fetal kidney; UBERON=UBERON_0002113. OX NCBI_TaxID=9606; ! Homo sapiens (Human) hi CVCL_D4BW ! HEK293T TetOff-SNCA-A53T SX Female AG Fetus CA Transformed cell line Parental cell line: HEK293T (RRID:CVCL_0063)
HEK293T TetOff-SNCA-A53T mRuby3-Galectin-3 mClover3-Galectin-3
By onID HEK293T TetOff-SNCA-A53T mRuby3-Galectin-3 mClover3-Galectin-3 AC CVCL_D4BX SY Syn10 Galectin-3 FRET CC Characteristics: Transduced with an all-in-one TetOff construct for the controllable expression of untagged SNCA p.Ala53Thr (from parent cell line). CC Characteristics: Additional expression of two plasmids encoding mRuby3- and mClover3-tagged LGALS3, enabling a FRET-based readout of endolysosome damage (Direct_author_submission). CC Transfected with: HGNC; 11138; SNCA (with p.Ala53Thr). CC Transfected with: HGNC; 6563; LGALS3. CC Transfected with: UniProtKB; P04483; Escherichia coli Tn10 tetR. CC Transfected with: UniProtKB; P06492; Human herpesvirus 1 VP16 (UL28) (with p.Met1_Arg360del). CC Transfected with: UniProtKB; Q8ISF8; Entacmaea quadricolor eqFP611 (modified to produce the bright RFP mRuby3). CC Transfected with: UniProtKB; P42212; GFP (with p.Ser30Arg, p.Tyr39Asn, p.Ser65Gly, p.Gln69Ala, p.Phe99Ser, p.Asn105Thr, p.Tyr145Phe, p.Asn149Tyr, p.Met153Thr, p.Gly160Cys, p.Val163Ala, p.Ile171Val, p.Thr203His, p.Ala206Lys = mClover3). CC Transfected with: UniProtKB; P0C2P0; Aspergillus terreus blasticidin-S deaminase (Bsd). CC Transfected with: UniProtKB; P00552; Transposon Tn5 neo. CC Transformant: NCBI_TaxID; 28285; Adenovirus 5. CC Transformant: NCBI_TaxID; 1891767; Simian virus 40 (SV40) . CC Miscellaneous: Cell line information from personal communication of Yuste-Checa P. CC Derived from site: In situ; Fetal kidney; UBERON=UBERON_0002113. OX NCBI_TaxID=9606; ! Homo sapiens (Human) hi CVCL_D4BW ! HEK293T TetOff-SNCA-A53T SX Female AG Fetus CA Transformed cell line Parental cell line: HEK293T (RRID:CVCL_0063)
HEK293T TetOff-SNCA-A53T
By onID HEK293T TetOff-SNCA-A53T AC CVCL_D4BW SY Syn10 CC Characteristics: Transduced with an all-in-one TetOff construct for the controllable expression of untagged SNCA p.Ala53Thr (Direct_author_submission). CC Transfected with: HGNC; 11138; SNCA (with p.Ala53Thr). CC Transfected with: UniProtKB; P04483; Escherichia coli Tn10 tetR. CC Transfected with: UniProtKB; P06492; Human herpesvirus 1 VP16 (UL28) (with p.Met1_Arg360del). CC Transfected with: UniProtKB; P0C2P0; Aspergillus terreus blasticidin-S deaminase (Bsd). CC Transfected with: UniProtKB; P00552; Transposon Tn5 neo. CC Transformant: NCBI_TaxID; 28285; Adenovirus 5. CC Transformant: NCBI_TaxID; 1891767; Simian virus 40 (SV40) . CC Miscellaneous: Cell line information from personal communication of Yuste-Checa P. CC Derived from site: In situ; Fetal kidney; UBERON=UBERON_0002113. OX NCBI_TaxID=9606; ! Homo sapiens (Human) HI CVCL_0063 ! HEK293T SX Female AG Fetus CA Transformed cell line Parental cell line: HEK293T (RRID:CVCL_0063)
HEK293T SNCA-A53T-mRuby3 SNCA-A53T-mClover3
By onTransfected with two plasmids encoding mRuby3- and mClover3-tagged SNCA p.Ala53Thr expressed under the control of CMV promoters.
HEK293E_Clu TL4
By onCell Line: Transfected with the pB-T-PAF expression plasmid expressing CLU under the tet operator and the PB-RN reverse transactivator plasmid expressing the reverse tetracycline-controlled transactivator under the CMV promoter.
HEK293E_Clu TL2+3
By onCell Line: Transfected with the pB-T-PAF expression plasmid expressing CLU under the tet operator and the PB-RN reverse transactivator plasmid expressing the reverse tetracycline-controlled transactivator under the CMV promoter.
HEK293E_Clu TL3
By onCell Line: Transfected with the pB-T-PAF expression plasmid expressing CLU under the tet operator and the PB-RN reverse transactivator plasmid expressing the reverse tetracycline-controlled transactivator under the CMV promoter.
HEK293E_Clu TL2
By onID HEK293E_Clu TL2 AC CVCL_D4BF CC Characteristics: Transfected with the pB-T-PAF expression plasmid expressing mutated CLU under the tet operator and the PB-RN reverse transactivator plasmid expressing the reverse tetracycline-controlled transactivator under the CMV promoter (Direct_author_submission). CC Transfected with: HGNC; 2095; CLU (with p.Tyr204Ser, p.Tyr206Ser, p.Leu207Ser, p.Phe209Ser, p.Leu211Ser, p.Phe218Ser, p.Phe219Ser and p.Phe220Ser). CC Transfected with: UniProtKB; P13249; S.alboniger pac (PuroR). CC Transfected with: UniProtKB; P04483; Escherichia coli Tn10 tetR. CC Transfected with: UniProtKB; P06492; Human herpesvirus 1 VP16 (UL28) (with p.Met1_Arg360del). CC Transfected with: UniProtKB; P03211; Epstein-Barr virus (strain B95-8) EBNA1. CC Transformant: NCBI_TaxID; 28285; Adenovirus 5. CC Miscellaneous: Cell line information from personal communication of Yuste-Checa P. CC Derived from site: In situ; Fetal kidney; UBERON=UBERON_0002113. HI CVCL_6974 ! HEK293-EBNA SX Female AG Fetus CA Transformed cell line Parental cell line: HEK293T (RRID:CVCL_0063)
HEK293E_Clu DD3
By onCell Line: Transfected with the pB-T-PAF expression plasmid expressing CLU under the tet operator and the PB-RN reverse transactivator plasmid expressing the reverse tetracycline-controlled transactivator under the CMV promoter.
HEK293E_Clu DD2
By onID HEK293E_Clu DD2 AC CVCL_D4BP CC Characteristics: Transfected with the pB-T-PAF expression plasmid expressing mutated CLU under the tet operator and the PB-RN reverse transactivator plasmid expressing the reverse tetracycline-controlled transactivator under the CMV promoter (Direct_author_submission). CC Transfected with: HGNC; 2095; CLU (with p.Tyr125Phe, p.Gly132Glu, p.Leu135Lys, p.Arg138Glu, p.Gln139Ala and p.Glu142Ser). CC Transfected with: UniProtKB; P13249; S.alboniger pac (PuroR). CC Transfected with: UniProtKB; P04483; Escherichia coli Tn10 tetR. CC Transfected with: UniProtKB; P06492; Human herpesvirus 1 VP16 (UL28) (with p.Met1_Arg360del). CC Transfected with: UniProtKB; P03211; Epstein-Barr virus (strain B95-8) EBNA1. CC Transformant: NCBI_TaxID; 28285; Adenovirus 5. CC Miscellaneous: Cell line information from personal communication of Yuste-Checa P. CC Derived from site: In situ; Fetal kidney; UBERON=UBERON_0002113. HI CVCL_6974 ! HEK293-EBNA SX Female AG Fetus CA Transformed cell line Parental cell line: HEK293T (RRID:CVCL_0063)
HEK293E_Clu DD1
By onCell Line: Transfected with the pB-T-PAF expression plasmid expressing CLU under the tet operator and the PB-RN reverse transactivator plasmid expressing the reverse tetracycline-controlled transactivator under the CMV promoter.
HEK293E_Clu CC4
By onID HEK293E_Clu CC4 AC CVCL_D4BM CC Characteristics: Transfected with the pB-T-PAF expression plasmid expressing mutated CLU under the tet operator and the PB-RN reverse transactivator plasmid expressing the reverse tetracycline-controlled transactivator under the CMV promoter (Direct_author_submission). CC Transfected with: HGNC; 2095; CLU (with p.Ser161Arg). CC Transfected with: UniProtKB; P13249; S.alboniger pac (PuroR). CC Transfected with: UniProtKB; P04483; Escherichia coli Tn10 tetR. CC Transfected with: UniProtKB; P06492; Human herpesvirus 1 VP16 (UL28) (with p.Met1_Arg360del). CC Transfected with: UniProtKB; P03211; Epstein-Barr virus (strain B95-8) EBNA1. CC Transformant: NCBI_TaxID; 28285; Adenovirus 5. CC Miscellaneous: Cell line information from personal communication of Yuste-Checa P. CC Derived from site: In situ; Fetal kidney; UBERON=UBERON_0002113. HI CVCL_6974 ! HEK293-EBNA SX Female AG Fetus CA Transformed cell line Parental cell line: HEK293T (RRID:CVCL_0063)
HEK293E_Clu CC3
By onCell Line: Transfected with the pB-T-PAF expression plasmid expressing CLU under the tet operator and the PB-RN reverse transactivator plasmid expressing the reverse tetracycline-controlled transactivator under the CMV promoter.
HEK293E_Clu CC2
By onCell Line: Transfected with the pB-T-PAF expression plasmid expressing CLU under the tet operator and the PB-RN reverse transactivator plasmid expressing the reverse tetracycline-controlled transactivator under the CMV promoter.
