Gene expression analysis
By onTo evaluate whether human microglia cells (hMG) can be activated by neuromelanin (NM), hMG were cultured with and without the presence of NM for 24 h and then harvested to measure gene expression of selected pro-inflammatory and neurotoxic factors.
SNP Genotyping and ApoE Genotyping
By onProtocol outlines DNA extraction from blood, quality control, SNP, and APOE genotyping. Adapted from PRoBaND SNP Genotyping and ApoE Genotyping Protocol by Malek et al. for Parkinson's Disease study.
Regulation of mitophagy by the NSL complex underlies genetic risk for Parkinson’s disease: Bioinformatic Prioritisation and Hit Validation
By onThis protocol describes the Bioinformatic Prioritization of PD GWAS candidates for High Content Screening, and Hit Validation by allele-specific expression (ASE) analysis.
Validation of Genotyping Method for L444P Mice Ear-Clips.
By on|| Team Schapira || Authors Revi Shahar Golan, David ChauAbstractAim: the genotyping is used to identify if mice are heterozygote (hetero) or Wild-Type (WT), and the aim of the work is to validate the digestion method, and PCR program, the PCR primers, and the interpretation of the results. Associated with publication: doi: 10.1093/brain/awx221
Glucosylceramidase Beta (GBA) Genotyping
By onThis protocol details the steps for GBA genotyping. This protocol has been adapted from the PRoBaND Clinical Consortium (incorporating methods described by Neuman et al., 2009 and Stone et al., 2000) and has been used for all publications for PRoBaND / Tracking Parkinson's describing clinical data and outcomes with respect to GBA status
Genotyping mice from ear clips
By onThis protocol describes the genotyping procedure from ear clip samples. This includes a general PCR protocol for primers with annealing temperatures in the range 55-70 degrees C. For other primers, the thermal cycling should be adjusted.
CRISPR/Cas9 genome editing
By onThis protocol describes the generation of DNAJC5 KO using CRISPR/Cas9 edition
