Genotyping Atp13a4(KO) mice

This protocol outlines the step-by-step process for DNA extraction from mouse biopsies and genotyping of Atp13a4(KO) mice using PCR. DNA is extracted from tail or ear biopsies using a simple alkaline lysis method followed by neutralization. PCR amplification is performed using specific primers for Atp13a4, and products are analyzed on an agarose gel to distinguish between wild-type and knockout genotypes.