Gavage – food supplementation for stalled weight gain in mice
By onFeeding protocol for Ndufs2fl/fl mice,
Mouse Stereotaxic Surgery
By onThis protocol describes the steps for performing stereotaxic surgery in mice. It is applicable to intracranial injections (e.g. virus, drug) and placement of implants (e.g. optical fibers, electrode arrays) into targeted regions of mouse brains.
Protocols for “Synaptotagmin-1-dependent phasic axonal dopamine release is dispensable for basic motor behaviors in mice”
By onThis is a collection of protocols for the manuscript "Synaptotagmin-1-dependent phasic axonal dopamine release is dispensable for basic motor behaviors in mice"
Deep Brain Stimulation (DBS) Implant
By onThis protocols describing the steps for making implants used for deep brain stimulation (DBS) in rodents.
Optogenetic Manipulation (Mouse)
By onThis protocol describes the steps for in vivo ontogenetic manipulation in mice, including assembly of fiber-ferrules, surgical implantation of fibers, and testing procedure.
Fiber Photometry (Mouse)
By onThis protocol describes the procedure for fiber photometry in awake behaving mice. It includes details on the surgical implantation of fibers.
Ex Vivo Electrophysiology
By onThis protocol describes preparation of brain slices, setup of electrophysiology rig, and solutions for collecting whole cell and cell attached recordings.
AAV injection in the nodose ganglia of mouse
By onThe gut-brain axis links the visceral organs to the medulla oblongata via the vagus nerve. Accessing to the afferent vagal pathway is important to dissect the role of cell populations in the bidirectional communication between brain and body. The jugular-nodose ganglion (JNG) complex contains the cell bodies of many heterogenous neural subpopulations responsible for sensing the physiological and pathological conditions of the thoracic and abdominal organs. However, the study of these ganglia is challenging in small animals due to size and location. Hence, in this protocol we describe a practical surgical approach to the vagal trunk, and the JNG complex in mice.
Standard Operating Procedure (SOP) for Combined ICA and Systemic Administration of MPTP: The Overlesioned (Bilateral Asymmetric) Non-human Primate Model
By onThis standard operating procedure (SOP) describes safe methods for the use of 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP) in non-human primates (adult macaque monkeys).
Generation of A549 PPM1H BromoTag CRISPR/CAS9 knock-in cell line
By onThis protocol details the generation of A549 PPM1H BromoTag CRISPR/CAS9 knock-in cell line.
Sleep-Wake Recording in Dan lab
By onSurgery and Setups for Recording 24-Hour Natural Sleep/Wake Cycles in Mice.
Sleep-Wake Recording in the Turek Laboratory
By onSurgery and recording protocol for measuring sleep-wake behavior in mice for Turek Laboratory.
Localised axotomy of human Cortical Neurons (CNs) from induced pluripotent stem cells (iPSCs)
By onThis protocol describes the process followed to perform localised axotomy of iPSC-derived human cortical axons cultured within fluid-walled dumbbells in 6 cm TCT-treated Petri dishes.
Viral-mediated short-hairpin RNA knockdown
By onStereotaxic injection of viral vectors for gene knockdown experiments: The AAV vectors for knocking down the gene of interest were custom-made with Vector Biolabs using AAV-GFP-U6 vector for AAV1 packaging (Cat no, 7040, Vector Biolabs).
Preparation and Administration of Systemic 4-AP
By onProcedure for preparation and systemic administration of 4-AP used in "Serine-129 phosphorylation of α-synuclein is an activity-dependent trigger for physiologic protein-protein interactions and synaptic function."
CRISPR nuclease (CRISPRn) genome editing
By onProcedure for CRISPR nuclease (CRISPRn) genome editing used in "Serine-129 phosphorylation of α-synuclein is an activity-dependent trigger for physiologic protein-protein interactions and synaptic function."
CRISPR knock-in (endogenous tagging)
By onProcedure for CRISPR knock-in (endogenous tagging) used in "Serine-129 phosphorylation of α-synuclein is an activity-dependent trigger for physiologic protein-protein interactions and synaptic function."
CRISPR knock-in validation
By onProcedure for CRISPR knock-in validation used in "Serine-129 phosphorylation of α-synuclein is an activity-dependent trigger for physiologic protein-protein interactions and synaptic function."
