Plasmid: V5-KANSL1 pLVX-EF1α-IRES-Puro
By onPlasmid: Human KANSL1 cDNA with N-terminal V5 tag cloned into the pLVX-EF1α-IRES-Puro plasmid (EcoRI and SpeI restriction sites) from Clontech, Takara Bio.
pCAG- WIPI2dC93E-cs- TEV-STREP
By onPlasmid: Mammalian expression of human WIPI2d C93E with C-terminal Strep.
cDNA clones for expression of LysoTag (TMEM192) in mammalian cells
By onPlasmids for expression of TMEM192 (LysoTag) in human and mouse cells.
pCAG-OSF-ATG13 (2-197)-K15D
By onPlasmid for expression of human ATG13 HORMA K15D mutant in mammalian cells.
pBPK1520-SNCA-A53T-ng
By onIt can be used to introduce SNCA-A53T mutation using prime editing, PE3 approach.
pCAG-ATG13 (1-197)
By onPlasmid: To make ATG101/ATG13 HORMA complex for expression in mammalian cells.
pU6-pegRNA-HEK3-CTTins-px330-scaffold
By onPlasmid: This resource can be used to introduce HEK3 CTT insertion using prime editing. It serves as a good positive control for prime editing.
pAC150-Keima-RAMP4
By onThe text describes the use of a PiggyBac vector to express the Keima-RAMP4 autophagy flux reporter, a tool for studying autophagy processes in cells.
