pCAG-MBP-Foldon-ATG9 (828-839)
By onPlasmid: To make ATG9 C-terminal tail trimer for expression in mammalian cells.
pCAG-MBP-ATG9 (692-839)
By onPlasmid: To make monomeric ATG9 C-terminal tail for expression in mammalian cells.
pLenti HsATP10B_D433N-T2A-His-flag-TMEM30A
By onPlasmid: Transfer plasmid for lentiviral vector production expressing Hs ATP10B D433N mutant and His/flag tagged Hs TMEM30A.
pGBdest-10xHis-TEV-ATG5_ATG7_ATG10_ATG12_ATG16L1_F32A-I35A-I36A_-GFP-TEV-StrepII
By onPlasmid: Plasmid for the Expression of the E3 complex with an FII (F32A, I35A, and I36A) mutation. SMC Internal reference: SMC1728
pCAG- WIPI2dK88E-cs- TEV-STREP
By onPlasmid: Mammalian expression of human WIPI2d K88E with C-terminal Strep.
pCAG-MBP-Foldon-ATG9 (801-839)
By onPlasmid: To make ATG9 C-terminal tail trimer for expression in mammalian cells.
pCAG- WIPI2dR108E- cs-TEV -STREP
By onPlasmid for mammalian expression of human WIPI2d R108E with C-terminal Strep.
pAC150-Keima-REEP5
By onSummary: Piggybac vector used to express Keima-REEP5 autophagy flux reporter, a tool to monitor autophagy flux in cells by measuring pH changes in autophagosomes and lysosomes.
cDNA clone for bacterial expression of human PPM1H H153D
By onPlasmid for bacterial expression of human PPM1H (H153D); contains TEV protease site for removal of 6His tag.
