Purification of FIP200 CTR-GFP
By onThis protocol describes how to express and purify GFP-FIP200 (CTR).
Purification of CK2 kinase complex
By onThis protocol describes how to express and purify CK2 kinase complex
Purification of Lambda Protein Phosphatase
By onThis protocol describes how to express and purify Lambda Protein Phosphatase
Purification of mCh-WIPI2d-IDR (364-426aa)
By onThis protocol describes how to express and purify mCh-WIPI2d-IDR (364-426aa)
Purification of GST-WIPI1/WIPI2d/WIPI3/WIPI4
By onThis protocol describes how to express and purify GST-WIPI1/WIPI2d/WIPI3/WIPI4
Purification of mCherry-WIPI2d/WIPI3
By onThis protocol describes how to express and purify mCherry-WIPI2d/WIPI3
Purification of mCherry- or GFP-tagged ATG13/101 subcomplex
By onThis protocol describes how to express and purify mCherry- or GFP-tagged ATG13/101 subcomplex
Purification of mCherry-ATG101/13 (1-191aa) subcomplex
By onThis protocol describes how to express and purify mCherry-ATG101/13 (1-191aa) subcomplex
Purification mCherry-ATG13 IDR
By onThis protocol describes how to express and purify mCherry-ATG13 IDR
Hutu80 and NCI h716 treatment with SCFAs and α synuclein Western Blotting
By onThis protocol describe the treatment of human enteroendocrine cell models Hutu80 and NCI h716 parental cells with SCFAs and GCase inhibitor (CBE) in order to detect the impact of SCFAs and GCAse inhibition on alpha synuclein protein expression by imm
Gene Expression protocol used in “LRRK2-G2019S Synergizes with Ageing and Low-Grade Inflammation to Promote Gut and Peripheral Immune Cell Activation that Precede Nigrostriatal Degeneration”
By onGene Expression protocol used in "LRRK2-G2019S Synergizes with Ageing and Low-Grade Inflammation to Promote Gut and Peripheral Immune Cell Activation that Precede Nigrostriatal Degeneration"
PFF seeding in U2OS cells
By onThis protocol describes the seeding of intracellular inclusions in U2OS cells harboring overexpressing a-synuclein via exogenous addition of short-length preformed a-synuclein fibrils.
Whole cell protein extraction and Western blotting
By onThis protocol describes cell lysis for whole-cell protein extraction followed by western blotting using LI-COR Odyssey quantitative western blot.
Expression and purification of Syp-VAMP2 complex into native nanodiscs
By onThis is a protocol for the purification of Syp-VAMP2 complex into native nanodiscs.