Purification of FIP200 CTR-GFP
By onThis protocol describes how to express and purify GFP-FIP200 (CTR).
Purification mCherry-ATG13 IDR
By onThis protocol describes how to express and purify mCherry-ATG13 IDR
Purification of mCherry-ATG101/13 (1-191aa) subcomplex
By onThis protocol describes how to express and purify mCherry-ATG101/13 (1-191aa) subcomplex
Hutu80 and NCI h716 treatment with SCFAs and α synuclein Western Blotting
By onThis protocol describe the treatment of human enteroendocrine cell models Hutu80 and NCI h716 parental cells with SCFAs and GCase inhibitor (CBE) in order to detect the impact of SCFAs and GCAse inhibition on alpha synuclein protein expression by imm
Gene Expression protocol used in “LRRK2-G2019S Synergizes with Ageing and Low-Grade Inflammation to Promote Gut and Peripheral Immune Cell Activation that Precede Nigrostriatal Degeneration”
By onGene Expression protocol used in "LRRK2-G2019S Synergizes with Ageing and Low-Grade Inflammation to Promote Gut and Peripheral Immune Cell Activation that Precede Nigrostriatal Degeneration"
Whole cell protein extraction and Western blotting
By onThis protocol describes cell lysis for whole-cell protein extraction followed by western blotting using LI-COR Odyssey quantitative western blot.
PFF seeding in U2OS cells
By onThis protocol describes the seeding of intracellular inclusions in U2OS cells harboring overexpressing a-synuclein via exogenous addition of short-length preformed a-synuclein fibrils.
Expression and purification of Syp-VAMP2 complex into native nanodiscs
By onThis is a protocol for the purification of Syp-VAMP2 complex into native nanodiscs.
Protocol for the production of RAB1A-10xHis in HEK293Gnti cells
By onProtocol for production of RAB1A-10xHis
Purification of GST-FAM134C
By onThis protocol details the purification of GST-FAM134C and its analysis.
Jess western blot automated system
By onBrief description of preparation of samples for Jess western blot automated system. Detailed instructions are included with the reagent pack
