Western blot for tissue extract
By onProtocol for the detection of proteins by Western blot from tissue extract.
Purification of NAP1 or GST-NAP1
By onThis protocol describes purification of GST-NAP1 and unlabeled NAP1.
Purification of MBP-NAP1
By onThis protocol describes the purification procedure of MBP-tagged NDP52 from E. Coli.
Calf intestine alkaline phosphatase (CIAP) treatment
By onProtocol selectively dephosphorylates non-aggregated PSER129, improving detection of aggregated PSER129, aiding in distinguishing between physiological and disease-related forms of alpha-synuclein.
Multiplex Labeling with Tyramide Fluorophores (Free-Floating Tissues)-Killinger Lab 2024
By onProtocol for multiplex labeling of free-floating tissues with tyramide fluorophores in the Killinger lab (2024).
Purification of Proteins from PFA Fixed Samples
By onProtocol for purifying proteins from PFA-fixed samples and extracting proteins from formalin-fixed tissues, including biotin pulldown before LC-MS/MS. Samples used for mass spectrometry, immunoblotting, and pulldowns.
Protocol for immunoperoxidase detection of alpha-synuclein in non-human primate brain samples
By onThe intraputaminal delivery of AAV9-SynA53T resulted in a widespread synucleinopathy throughout the cerebral cortex and the substantia nigra in the non-human primate brain
Protocol for quantification of the nigrostriatal system in non-human primate brain sections
By onThe intraputaminal delivery of AAV9-SynA53T resulted in a widespread synucleinopathy throughout the cerebral cortex and substantia nigra in the non-human primate brain
Aggregation of human recombinant alpha-synuclein
By onThis protocol describes a method of generating aggregation of human recombinant alpha-synuclein.
α-Synuclein Protein Preparation (Large scale)
By onProtocol for large-scale preparation of recombinant α-synuclein protein.
