Proteomics workflow for whole cell lysate, endosome, and lysosome fractions
By onA protocol for sample preparation for LC-MS analysis of whole cell lysates and for lysosomal and endosomal fractions purified by Lyso-IP and Endo-IP.
In vitro assembling of RNP for nucleofection of hPSCs
By onThis protocol describes the procedure for the in vitro assembly of ribonucleoprotein (RNP) which can be delivered into human pluripotent stem cells (hPSCs) using nucleofection.
Expression and purification protocol of GST-mCh-FYVE
By onThis protocol details the expression and purification of GST-mCh-FYVE.
Expression and purification protocol of WIPI2d
By onThis protocol details the expression and purification protocol of WIPI2d.
Expression and purification of PI3KC3-C1 complex
By onProtocol describing expression and purification of PI3KC3-C1 complex.
NAP1-mCherry
By onThis protocol describes how to express and purify human NAP1 tagged C-terminally with mCherry.
Expression and purification protocol of GST-TAX1BP1
By onThis protocol details the expression and purification protocol of GST-TAX1BP1.
Insect Cell Protocol for LRRK1 and LRRK2 Expression
By onProtocol for expressing LRRK1 and LRRK2 in insect cells.
Western blot protocol for detecting ATP10B in mouse/rat brain
By onProtocol for detection of ATP10B in rat and mouse brain tissue by Western blotting.
Assessing enrichment of proteins in the mitochondrial fraction in HEK cells
By onThis is a method for measuring protein enrichment on mitochondria in various conditions. In the resulting Western blot, one can assess the level of contamination of other organelles in the enrichment prep.
Expression and purification GST-tagged ATG13:ATG101 constructs
By onExpression and purification of GST-tagged ATG13/ATG101 constructs.
SINTBAD-GFP: expression and purification
By onThis protocol describes how to express and purify human SINTBAD tagged C-terminally with eGFP.
NDP52 and OPTN S177D S473D: expression and purification
By onThis protocol describes how to express and purify human NDP52 and OPTN S177D S473D. The same procedure can be applied to purify wild type OPTN.
Whole mount dissection and staining of enteric nervous system
By onThis protocol details whole mount dissection and staining of the enteric nervous system.
Immunoprecipitations and Western blots analysis
By onProtocol for immunoprecipitation and Western blotting as performed in "Synapsin E-domain is essential for α-synuclein function." (https://doi.org/10.7554/eLife.89687.1)
GST fusion protein production
By onProtocol for GST fusion protein production as performed in "Synapsin E-domain is essential for α-synuclein function." (https://doi.org/10.7554/eLife.89687.1)
Purification of recombinant Low Density Lipoprotein Receptor Related Protein Associated Protein 1 (LRPAP1, RAP) from Escherichia coli
By onThis protocol details how to efficiently purify LDL Receptor Related Protein Associated Protein 1 (LRPAP1 or RAP) from Escherichia coli.
GFP-Clu-tails purification from Escherichia coli cells
By onThis protocol details how to purify the fusion protein GFP-Clu-tails from Escherichia coli.
MBP-Clu-tail purification from Escherichia coli cells
By onThis protocol details how to efficiently purify the fusion protein Maltose binding protein (MBP)-Clu-tail (204-238) from Escherichia coli.
Expression and Purification of RH proteins via MBP affinity tag
By onAffinity purification followed by size exclusion chromatography.
