Testing AAVrg-hSyn-mCherry in the Spinal Cord of Rhesus Macaques
By onViral Vector: Two rhesus macaque animals were tested for AAVrg-hSyn-mCherry in the spinal cord. The authors observed abundant mCherry-positive neurons in expected targets (i.e., motor cortical regions known to project to the spinal cord).
Testing AAVrg-hSyn-mCherry in the Putamen of Rhesus Macaques
By onTwo rhesus macaques were tested with AAVrg-hSyn-mCherry in the putamen, showing numerous mCherry-positive neurons in motor cortical regions projecting to the putamen.
pAAV_Cbh_DIO_hTyr
By onCre-dependent expression of human tyrosinase induces the formation of neuromelanin in catecholaminergic neurons. The CbH promoter is a strong, pan-neuronal, non-silencable promoter.
pAAV_Ef1a_DIO_hTyrHA_minBack
By onCre-dependent expression of human tyrosinase induces the formation of neuromelanin in catecholaminergic neurons. Expression under strong Ef1a promoter.
pAAV_Ef1a_DIO_hTyrNull_HATag
By onStrong expression of Ef1a promoter, HA tag for histological verification of expression.
pAAV_Cbh_DIO_hTyrNull_HATag
By onStrong expression of CbH promoter that is non-silencenable, HA tag for histological verification of expression.
pAAV_EF1a_DIO_jGCaMP8f_nano_ribo_WPRE
By onThis links GCaMP8f with a ribosome-tethered nanobody that restricts GCaMP to the soma of neurons. Useful in very dense nuclei with strong neuropil, which can make post-hoc cell segmentation difficult.
pAAV_Ef1a_DIO_MyrPalm_mScarlet_WT_IRES_mNeon_minBack
By onBright green (mNeon) and red (mRuby) fluorescence proteins are expressed, with the former being cytoplasmic and the latter membrane-targeted. This expression is ideal for tracking long diffuse axons in cortical structures in vivo or for slice histology and cleared brain tissues post-mortem. The construct is expressed under a strong EF1a promoter in a cre-dependent manner.
pAAV_hSyn_GCaMP6m_sWPRE_spA_mDLX_nls_mRuby3_ssWPRE_spA
By onIt is a viral construct designed to express GCaMP6m pan-neuronally and specifically express a red fluorophore (mRuby) in the nucleus of cortical interneurons. This construct is ideal for dual-imaging experiments to capture neuronal activity of two neuronal populations with the sensitive blue-absorbing GCaMP6m (no light artifact) and to identify interneurons with red fluorescence in parallel or post-mortem.
pAAV_CbH_SIO_SyGCaMP8s_p2a_mRuby
By onThis construct links GCaMP8s to Synaptophysin and directs the fusion protein to presynaptic sites. It also produces a cytoplasmic red fluorescence protein (mRuby) via the self-splicing peptide 2A.
Ef1a-DIO-PSAML-141F,Y115F-GlyR-2A-FusionRed plasmid
By onPharmacologically Selective Actuator Module, chimeric ligand-gated ion channels that can be used to manipulate electrophysiological activity.
