DNA and Expression Following Nucleosome Depletion (DEFND) Sequencing on Cryopreserved Colon Tissue

By Kathy Plaisance, Antonina Mikorska, BSc and Thierry Voet, PhD
View Published Protocol

Output Details

Description
This protocol details DNA and Expression Following Nucleosome Depletion (DEFND) sequencing optimized for cryopreserved colon tissue.
Tags
  • Sequencing
Team
Team Voet
Program
Collaborative Research Network
Theme
PD Functional Genomics

Meet the Authors

Related Research

Single cell analysis of iPSC-derived midbrain organoids

The following script was used for analysis of gene corrected (GC) versus GBA1 mutant (MUT) midbrain organoids. The purpose was to combine, filter, integrate, and identify clusters and differentially expressed genes sets. This is part of a…

View Code

Glucosylceramidase Beta (GBA) Genotyping

This protocol details the steps for GBA genotyping. This protocol has been adapted from the PRoBaND Clinical Consortium (incorporating methods described by Neuman et al., 2009 and Stone et al., 2000) and has been used for all publications for…

View Protocol

Detecting Full-Length EccDNA with FLED and long-reads sequencing

Reconstructing the full-length sequence of extrachromosomal circular DNA (eccDNA) from short sequencing reads has proved challenging given the similarity of eccDNAs and their corresponding linear DNAs. Previous sequencing methods were unable to…

View Article
View More Outputs
Aligning Science Across Parkinson's
Powered by  GDPR Cookie Compliance
Privacy Overview

This website uses cookies so that we can provide you with the best user experience possible. Cookie information is stored in your browser and performs functions such as recognising you when you return to our website and helping our team to understand which sections of the website you find most interesting and useful.